RNA-Seq refers to the use of high throughput next generation sequencing technologies to sequence complementary DNA (cDNA) sequences. Successful whole transcriptome analysis depends on RNA quality and efficient conversion into cDNA libraries. The entire pool of RNA present in the sample is converted into cDNA copies, sequenced and mapped on to a reference genome. Xcelris Genomics helps you with the best solutions for transcriptome sequencing on various platforms like Illumina MiSeq, NextSeq500, HiSeq 2000,/2500 SOLiD 4, Ion Torrent Life Technologies and Roche GS FLX Titanium etc. These technologies offer powerful combination of read length and fragment or paired end library flexibility for transcriptomes of various sizes. This allows profiling of the whole population of mRNA and enables mapping and digital quantification of whole transcriptome. We have successfully established accurate and comprehensive methodologies for animal, plants, bacteria, fungi and many more. "Barcoding/Indexing" can be carried to allow RNA Seq of multiple samples in single run/lane/slide. Analysis of the reads generated by the sequencer depends on presence or absence of reference genome. In non-model organisms, the data generated is assembled using the denovo approach. The reference based approach is preferred for model organisms whose genomes are available along with proper annotations.