Medical Genetics Services
Genotyping By Sequencing(GBS)/
Restriction site Associated DNA Sequencing(RAD)
GBS/RAD‐seq is a method that uses Illumina HiSeq2000/ 2500 next generation sequencing platform to discover and score tens to hundreds of thousands of SNP markers in hundreds of individuals in model or non model organisms. It is a "complexity reduction" technique designed to reliably interrogate a segment of genome, the method involves cutting down a genome anywhere from 0.1 to 15% with at least one restriction enzyme and sequencing the ends of the resulting fragments for either genetic marker discovery or genotyping. It allows you to interrogate a scalable number of loci even in a highly heterozygous species that leads to study of QTL mapping and population genomics. GBS enables "deep sequencing" of RAD‐tags at 30‐60 x coverage to discover SNPs, giving researchers confidence that technology can identify genetic variation linked to a trait or population. The complexity of the genome is reduced by digesting the DNA with optimized enzymes. The most commonly used enzymes in plants are PstI, SbfI, SgrAI, ApeKI etc, and in animals are SbI, PstI, NotI, Eag1, BamHI, BbvC1 etc. Combination of enzymes set can be used to prepare libraries for parental as well as progeny lines and sequencing on HiSeq 2000/2500 platform using paired‐end or single‐end library can be carried out by multiplexing for 12, 24, 48, 96, 192 samples or as per request.

Xcelris Genomics has executed many projects on SNP genotyping by GBS. Xcelris Genomics has developed expertise in the field of GBS: SNP Discovery, association analysis, LD analysis and haplotyping of closely related markers using various tools & software's (including Bowtie, Stacks, RAD tool, JoinMap, TASSEL etc). GBS based on the Illumina HiSeq 2000 platform has proven to be a fast and cost-effective means of SNP discovery.
Whole Genome Sequencing   |    Whole Transcriptome Sequencing / RNA-Seq   |    Small RNA Sequencing
Genotyping By Sequencing(GBS)   |    Metagenomics and Metatranscriptomics Services
Mitochondrial DNA Sequencing   |    Tilling and Eco-Tilling   |    Genome Database Development Service
Exome Sequencing Service   |    eQTL
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