Medical Genetics Services
Tilling and Eco-Tilling
TILLING (Targeting Induced Local Lesions in Genomes) is a method in molecular biology that allows directed identification of mutations in a specific gene. TILLING was introduced in 2000, as functional genomic tool for the model plant Arabidopsis thaliana. TILLING is a nontransgenic technology that utilizes a reverse genetics approach for the production and detection of mutation and has been successfully employed in zebrafish, maize, wheat, rice, soybean, tomato, lotus, mustard, and lettuce. It overcomes the limitation of knockout experiments. TILLING combines a standard and efficient technique of mutagenesis with a chemical mutagen such as ethyl methanesulfonate (EMS) with a sensitive DNA screening technique that identifies single base mutations (also called point mutations) in a target gene. In contrast, Eco‐TILLING is a variation of TILLING that investigates the natural variation among cultivar/inbred line/accession when aligned with a sequenced reference genome for the identification of SNPs. Next‐generation sequencing (NGS), with advantage of high throughput sequencing of the targets provides advantage over the traditional LICOR gel based system to identify mutations involving PCR amplification and digestion of mismatches using the restriction enzyme Cel‐I. Drawbacks to the LICOR system is having to pour slab gels and long run times (~4 hours). The current NGS technologies are robust and convenient data analysis using bioinformatics tools to analyze thousands of samples at a time using different pooling schemes. Xcelris Genomics offers the high‐throughput of next‐generation sequencing technology of HiSeq 2000/2500, NextSeq500 and MiSeq along with resolving power of overlapping pool design for efficient and economical functional genomics analysis across thousands of TILLING population generated for different organisms.
Whole Genome Sequencing   |    Whole Transcriptome Sequencing / RNA-Seq   |    Small RNA Sequencing
Genotyping By Sequencing(GBS)   |    Metagenomics and Metatranscriptomics Services
Mitochondrial DNA Sequencing   |    Tilling and Eco-Tilling   |    Genome Database Development Service
Exome Sequencing Service   |    eQTL
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