Cat No.: SEQ-02

Process:
•   r-E. coli culture containing plasmid DNA, propagation in test tubes or in 96 deep well culture plates.
•   Plasmid DNA isolation (with XcelGen kit) and its quality check.
•   Sequencing of DNA with client specific primer or universal primer using Sanger sequencing
     chemistry.

Sample Requirement:
E.coli culture - 96-well bacterial culture in 200μl LB media /antibiotic markers /culture plates/ glycerol stock, details of inserted gene size and gene information, vector details, customer specified primers, conc.= 10 pmole/μl ( μM), volume- 10 μl and Tm of the primers.

Turn Around Time:
5-6 working days from the receipt of sample to Xcelris premises.

Deliverables:
Q20 read length up to 800 bases with ABI or SCF, FASTA file.