Medical Genetics Services
 
 
 
r-E.coli Culture Sequencing Services
Cat No.: SEQ-02

Process:
•   r-E. coli culture containing plasmid DNA, propagation in test tubes or in 96 deep well culture plates.
•   Plasmid DNA isolation (with XcelGen kit) and its quality check.
•   Sequencing of DNA with client specific primer or universal primer using Sanger sequencing
     chemistry.

Sample Requirement:
E.coli culture - 96-well bacterial culture in 200μl LB media /antibiotic markers /culture plates/ glycerol stock, details of inserted gene size and gene information, vector details, customer specified primers, conc.= 10 pmole/μl ( μM), volume- 10 μl and Tm of the primers.

Turn Around Time:
5-6 working days from the receipt of sample to Xcelris premises.

Deliverables:
Q20 read length up to 800 bases with ABI or SCF, FASTA file.
 
 
 
Plasmid/PCR Sequencing Services   |   r-E.coli Culture Sequencing Services   |   Primer Walk Sequencing
Microsatellite Genotyping   |   SNP Genotyping by Sequencing on ABI3730xl   |   SNP Genotyping by SNaPshot Assay
Microbial identification services   |   Customized Services
 
 
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