Fields marked with (
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Client Details:
Client Name:
*
E-mail:
*
Organization:
*
Contact No.:
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Address:
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1000
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Sample Details:
Number of Samples/Reactions/Data point:
*
Platform:
3730xl DNA analyzer
Application:
*
Sanger Sequencing
Genotyping by Sanger Sequencing
Gene Expression Analysis (RTPCR)
Sample Type:
*
Tissue
Culture
DNA
PCR product
Plasmid
RNA/cDNA
Service Required:
*
(1)
SEQ-1: Purified Plasmid/PCR Product Sequencing.
(2)
SEQ-4: Primer Walk sequencing.
(3)
MID-S: Sequencing data of 16S, 18S, 28S and ITS region.
(4) SNP GENOTYPING: SnaPshot Assay.
Client is providing Ready to Run plate
Client is providing Amplicon
Client is providing DNA samples
SNP information
Level of Multiplexing Information
Labelled Dye information
Amplicon Size information
Amplicon size information
SNP Information per sample
Sequence of amplicon in which SNP is to be detected with SNP location
Refernce Sequence
Quality Report of Isolated DNA
Primer information for amplification of locus in which SNP is to be identified
SNP information per sample
Deliverables required by client:
Raw data files only
Raw data with SNP analysis report cosist of SNP calling peaks with standard curve and SNP list
(5) Gene Expression Analysis: SYBR Green assay or Taqman Assay
Client is providing tissue
RNA samples
RNA Intigrity Number should be above 6
Client want to perform absolute quantification
Relative quantification
Client is providing control sample
Gene Information both
Test Gene, House-keeeping gene with primer information
Validated conditions for amplification of Test and House-keeeping gene
Deliverables required by client:
Raw data files only
Complied summary report consist of delta-delta CT value, fold changes value, melt curve and total protocol performed report
(6)
SEQ-2: recombinant E-coli clone sequencing.
(7)
MID-R: Microbial identification of Bactreia/Yeast/Fungus with MID report.
(8)
MID-C: Microbial identification of Bactreia/Yeast/Fungus with Contig report.
(9) Microsatellite Genotying on ABI 3730XL.
Client is providing Ready to Run plate
Client is providing Labelled Amplicon
Client is providing DNA samples
Allelic information
Level of Multiplexing
Dye used for Multiplexing
Amplicon size information
Allelic Information
Flouroscent Labelled Dye information
Multiplexing Information
Amplicon size information
Quality Report of Isolated DNA
Primer information for amplification with standardized condition for each primer
Sizing information
Deliverables required by client:
Raw data files only
Raw data and compiled report consist of allelic information status of sample with peak area and Peak
Extra Service Required:
ExoSAP-06: Enzymetic Purification of PCR product
Gel-Extraction: Purification of PCR product from Agarose gel
Additional Informatiom from Client for the Experiment:
(Maximum no. of chars left :
1000
)
Estimated Budget:
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1000
)
Remarks:
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1000
)